Healing/actin peptide, almost always stacked with BPC; tumour-metastasis biology is a concern.
SDKPDMAEIEKFDKSKLKTETQEKNPLPSKETIEQEKQAGESthymosin β4, 43 aa, N-acetyl; commonly sold 'TB-500' is often the Ac-LKKTETQ (17-23) fragment, not the full protein
#31 of 61 research peptides · updated
Access
Grey market
Cost / month
≈ €15–73
at 140–710 mcg/day · Semax Polska · €3.39/mg
InjectionFelt in 7 days – 3 weeks
15 min1 h6 h1 day1 week1 month
Builds over days or weeks of regular use rather than with each dose.
2 weeks ago today I injured my shoulder doing bench. Massive bruise, couldn't sleep. No benching. Today I am doing CGBP with 225 for reps pain free and threw up a single at 255. No. Pain. BPC-157/TB500 FTW. 750 mcg daily.
ive been running 1mg each of bpc-157 and tb-500 now for 3 weeks for tennis elbow what i think to be a possible rotator cuff tear and buldging discs in my neck. i have felt zero relief for any of the above to this point
Nothing1 mg each of BPC-157 and TB-500 · 3 weeks
tennis elbow, possible rotator cuff tear, bulging neck discs
Strict case
Tβ4 has a well-documented pro-angiogenic and pro-metastatic role in cancer biology, and the fragment actually sold has no human safety data.
Users report
Rarely used alone, so praise is mostly for the BPC+TB stack: injuries back in weeks instead of months, chronic knee/shoulder pain gone. Solo users are less consistent, and some report TB-500 made a tendinopathy worse or caused emotional blunting. One user who carried on with TB-500 alone after stopping BPC woke with hives, a closing throat and swollen lips and needed an ambulance; others report flu-like first-dose reactions, months of headaches or leg swelling, which they blame on contaminated vials as often as on the peptide. Vendors mostly sell the 7-aa fragment while the research is on full Tβ4.
Best effects
Faster soft-tissue recovery · less chronic joint pain · flexibility / range of motion
Tβ4 promotes tumour cell migration, angiogenesis and metastasis in models and is overexpressed in many cancers · TB-500 fragment has zero human exposure data (FDA) · Generic: grey-market endotoxin; product often isn't the molecule studied
Evidence
Small human studies — For TB-500; small human trials of full Tβ4 (eye drops, IV phase 1)
Typical use
2-2.5 mg twice weekly for 4-6 weeks loading, then 1-2 mg weekly. Users report 500–570 mcg/day (median 500 mcg, n=9).
Getting it
Research-peptide vendors only. Many vials sold as 'TB-500' test as full-length Tβ4 (about 4921 Da) and vice versa. Covered by the Oct 2026 HHS enforcement discretion; banned in sport by WADA.
Stacked with
BPC-157: The 'Wolverine' stack; most TB-500 use is this pair, often as a pre-mixed vial. · GHK-Cu and KPV: 'GLOW' and 'KLOW' blends add these for skin and anti-inflammatory effects. · Growth hormone or GH secretagogues: Added by lifters for post-surgery healing; several said HGH did more than the peptides.
2 weeks ago today I injured my shoulder doing bench. Massive bruise, couldn't sleep. No benching. Today I am doing CGBP with 225 for reps pain free and threw up a single at 255. No. Pain. BPC-157/TB500 FTW. 750 mcg daily.
No side effects for me. Had bad tennis elbow for almost a year that was significantly affecting my workout routines. After 1 month of TB-500/BP-157 daily pain completely gone.
I used BPC-157 and TB 500 (known as the “Wolverine Stack”) to fix my chronic back pain (I’m also a retired disabled veteran). It prevented me from having major back surgery. I can now squat 405 (5x5 workout program) after a 10 year “hiatus” from powerlifting.
Glowing
chronic back pain, disabled veteran; Wolverine stack
After running BPC157 and TB500 for 3 weeks, my shoulder pain (which has bothered me for over a year) has absolutely vanished and gives me no pain while doing pressing movements anymore. None! I'm not sure if this is placebo or if it's legit
On Sept 18, I started injecting BCP-157 and TB-500 into the dorsum of my foot. Today, 9th Oct, I've been pain-free for over a week and I'm focusing on correcting my gait.
GlowingInjection · 3 weeks
old ACL tear and foot nerve damage; injected locally with BPC-157
I healed both an AC Joint separation (grade1) and a badly torn meniscus in about 4 weeks by taking subq injections of BPC-157/TB-500 at home. Bit of a learning curve but worth it 100% in my experience.
Doctor told me 3 months minimum before I could go back to work light duty. I went back 3 weeks later and the Dr was perplexed. I was 80% healed and he asked what I did.
Every doctor told me that my knee would probably ache and give me pain every day for the rest of my life unless I had surgery, and even then it would not be at 100%…. I haven’t had any problems with it for the last year.
Glowing
knee injury doctors said needed surgery; with BPC-157 (full-length TB4)
I did BPC alone for a week and it did nothing, added TB (2.5 mg to 3.5 mg every 3 days) and the pain was gone in 2 days. Ran it for 2 months then been off it for 3 weeks now. No pain for the first time in over 4 years.
i havnt been able to crawl.on my knees for 10yrs without feeling like 2 knifes stuck in each after 3 days on wolverine stack theres no pain and i can get up without using my hands
I used BPC157 and TB500 after my 2 level lumbar fusion. I am a neurosurgeon, reasearched as much as I could. I think it definitely sped up my recovery.
Positive
neurosurgeon, post two-level lumbar fusion with psoas hematoma; with BPC-157
This year I ran BPC-157 and TB-500 for a biceps and shoulder injury from the gym, and it helped the injury. A few weeks later I noticed the tinnitus was barely there. Still present, but at a level that doesn’t ruin my life anymore.
Positive~6 weeks
gym biceps/shoulder injury, chronic tinnitus; with BPC-157
It really works, especially fast for acute stuff. For fresh injuries, pain, and discomfort, I noticed relief quickly. Even chronic issues improved, though slower and less dramatically.
Positive500 mcg twice weekly at first (BPC-157 250 mcg/day) · Injection · 3 months
separate vials; high blood pressure and tachycardia on medication
But the general mobility improvement, training recovery improvement, better digestion, slightly better sleep make up for those initial days. My body actually felt younger after the protocol.
Positive250 mcg 2x/day each with BPC-157 · Injection · 50 days
Did the pain go away immediately after 4 weeks? No but it improved drastically. After a few months, I was back to doing shoulder and chest presses with no pain.
Positive500 mcg/day each with BPC-157 · Injection · 4 weeks
I've been using tb500 and bpc157 for months no hair growth,however increase recovery less inflammation,I feel really good more energy etc..but definitely my bald head of hair still has no hair
I used bpc157 and tb500 for a painful elbow injury. I'm 54 and experience occasional sudden knee weakness. That went away for about 6 months after 2 weeks of using those peptides.
I used a BPC-157/TB500 spray topical on a chronic elbow injury(small tear/tendinitis). I went from not being able to do bicep hammer curls at all to doing them pain free in 4 weeks.
As a very active 48y/o (BJJ, weights, touch rugby and former rugby union player) my joints, knee and back are feeling surprisingly better one month into using it.
For healing my rotator cuff and elbow tendonosis I took 4mg the first day then tapered down 30% every other day for 2.5 weeks. The first few cycles of Wolverine stack, I followed the .2-.5mg protocol and it did jack shit.
Mixed4 mg day 1, tapered over 2.5 weeks · 2.5 weeks (after several low-dose cycles)
I've been doing it for a little over 2 weeks but I will not be doing any shots today because I'm concerned about the anhedonia affects I've been getting (low libidio, flat mood, no motivation).
Mixed250 mcg 2x/day (Wolverine mix) · Injection · 2+ weeks
I used BPC-157 and TB-500 for my knees for two months. I noticed a slight improvement, but it really messed with my sleep. I started waking up several times during the night, and my HRV dropped quite a bit.
Been dealing with slight tear in my meniscus that creates irritation and swelling in my knee for about a year now. I’ve did bpc157/tb500 for about a week and swelling did go down but soreness remained.
I had some pretty good results with tb500/bc157 combination. Worked well for my knee, didn’t work for my shoulder (multiple tears, so not surprised it didn’t help)
ive been running 1mg each of bpc-157 and tb-500 now for 3 weeks for tennis elbow what i think to be a possible rotator cuff tear and buldging discs in my neck. i have felt zero relief for any of the above to this point
Nothing1 mg each of BPC-157 and TB-500 · 3 weeks
tennis elbow, possible rotator cuff tear, bulging neck discs
FWIW I’ve tried two courses of common peptides now (BPC-157 and TB-500), both 3 months at typical dosing, and at most I experienced some very small benefit from the latter (p=high)
I started the Tb500 first at 25units /500mcg and instantly had a negative side affect. Basically made me emotionally numb for a few hours , and any sensation positive or negative in my body was basically turned to zero.
Negative500 mcg · Injection
TB-500 alone, first dose; clinic-supplied vials later found to be past date
I injected the low starting dose Wednesday and within 2 hrs afterwards I had extreme dizziness, a terrible migraine, upset stomach, and couldn’t handle any stimulation.
Bad reactionNeuroprotection · Injection
6 years after a TBI; first low dose of BPC-157 with TB-500; mostly back to baseline after 4 days
I took my last dose of BPC-157/TB-4 (oral form) back in May, and the headaches have been ongoing ever since. Fortunately, the intense eye pain I initially had cleared up after about two weeks, but the headaches remain.
now it has been little over four weeks that I stopped tb500 and little over 2 months of continuous swelling and my feet, ankles and shins are still swollen 24/7.
Bad reactionInjection
third course; foot soft-tissue injury; continued TB-500 alone for a week; bloods and MRI normal
Not my proudest moment, but after BPC-157 and TB-500 from the gray market, I got hives all over my body. No issues later on with BPC from a different gray market or sermorelin from a legit c.p.
Bad reaction
grey-market BPC-157 + TB-500; no reaction later to BPC-157 from another source
Safety
Tier by safety barStrict ExcludedBalanced ExcludedEvidence only Caution
Each bead is one amino acid in the peptide's chain, coloured by its side chain: grey for non-polar, cyan for polar, blue for positively charged and fuchsia for negatively charged. Length and mix tell related peptides apart at a glance, from a two-residue dipeptide like carnosine to a 43-residue protein like thymosin beta-4. The colours describe chemistry only and predict neither effect nor safety.
non-polarpolarpositivenegative
Report grades
Every first-hand Reddit or X report is graded on its own, from glowing through positive, mixed, nothing and negative to bad reaction, and the bar shows how those grades split. Posts that look vendor-adjacent stay on the peptide's page but are left out of the counts.
GlowingPositiveMixedNothingNegativeBad reaction
% positive
The share of a peptide's graded Reddit and X reports that are glowing or positive, vendor-adjacent posts left out. A peptide with few reports is pulled toward the average of all reports, as if it had eight average ones besides its own, so two glowing posts cannot top the list. Kept peptides rank by the same reports' mean grade, shrunk the same way, and excluded ones follow in that order.
Researcher estimate
No first-hand report of this peptide has been graded yet, so the figure is the researchers' own rating out of 10 from what they read about it. It ranks alongside the computed scores until reports arrive.
Safety tier
Each peptide is tiered clean, caution or excluded from its five risk axes under the chosen safety bar. An excluded peptide has a plausible route to serious harm, so it stays listed with the reason but ranks behind every other entry, however good its reviews.
Safety bar
The safety bar sets how much risk it takes to exclude a peptide. Strict: out on any serious mechanism with little human data, or any documented serious harm. Balanced: out on documented serious harm (unless it is obvious, reversible and from a regulated product), or a serious mechanism whose harm would be silent with no human data at all. Evidence only: out only on documented serious harm in humans that is lasting, delayed or from grey-market product.
Seen harm
Documented serious adverse events in humans, from 0 for none despite real exposure to 3 for deaths or several well-documented serious harms. Serious means a hospital or emergency visit or harm lasting weeks, so a headache or a bad night does not count.
Mechanism
A plausible biological route to serious harm at the doses people use, such as growth-factor signalling, hormone-axis suppression, heart or blood-pressure effects or immune activation. It runs from 0 when none is known to 3 when a dangerous pathway has been shown in living animals.
Data gap
How little human safety data exists for the molecule actually sold, from 0 for an approved drug with large trials and years of use to 3 for no human safety data at all. A modified analogue does not inherit its parent's trials.
Silent harm
Whether the feared harm would show quickly or stay hidden, from 0 when it is obvious and goes on stopping to 3 when it is silent and delayed, like tumour promotion or slow organ damage. User reports cannot reveal a harm that takes years to appear, so a high score here limits what good reviews can tell you.
Product
The risk of what people actually buy, from 0 for a regulated pharmacy or supplement product taken by mouth or on the skin to 3 for a grey-market injectable. In one dataset of 6,441 grey-market samples, 42–71% failed basic quality criteria and 15% carried endotoxin.
Evidence
The strongest kind of study behind the peptide's effects, from an approved drug through human controlled trials and small human studies to animal work or nothing of its own. Animal results often fail to carry over to people.
Access
How people get it: as an approved drug, a compounded prescription, an over-the-counter supplement or from the grey market, where nobody checks the vial. The channel decides both the legal position and the risk of a bad batch.
Price per month
Thirty days of the typical dose, from its low end to its high end, priced at the listing a careful buyer would choose: in stock, from a shop scoring 4 of 5 on trust (third-party tested where no score is set), failing that 3, a pure pack before a blend, a European shop first, then the cheapest per mg. The range is the dose's, not the shops'. A per-dose amount counts once a day unless the dose says otherwise. Prices are in euros at ECB rates of 2026-10-29, before shipping; the shop table keeps each listing's price per mg, per gram for bulk supplements. Where no listing can be priced the researchers' own estimate stands in, and says so.
Dose users report
The middle half of the daily doses stated in at least five first-hand reports, vendor posts left out. Where its middle sits more than twice above or below the declared dose, the month is priced at it as well, since what people take is what they pay for.
Testing
Whether the shop publishes lab results: third-party tested means an independent lab checks each batch, own COA means the shop's own certificate of analysis, and no public tests means neither. A certificate shows purity, not safety.
Added peptide
Some packs sold under one peptide's name hold a second one, such as 5 mg GHK-Cu in a nasal kit, stated only in the description. Sprayed in the nose or injected it reaches the blood like an injection, so it carries the risk of that route and is tiered as the injectable form, whatever its tier on the skin. A pack whose page gives no contents list may hold more than its name.